CBLS WETLAB TANK TITRATIONS- BLUE & DISPLAY TANKS

Protocol Link from Personal Notebook and from Putnam Lab

Titrations of total 5 samples from CBLS Wetlab Blue Tanks (4) and Display Tank (1) along with 2 Junks and CRMs respectively.

Sample Information:

Date	Sample ID	Weight (in g)	Salinity (in psu)	Tank Type	Notes
20250819	BT 1	60.047	35.14	Blue Tank 1
20250819	BT 2	59.805	35.14	Blue Tank 2
20250819	BT 3	59.957	35.13	Blue Tank 3
20250819	BT 4	60.221	35.13	Blue Tank 4
20250819	DT 1	60.130	35.63	Display Tank

pH Calibration

• Following are the pH calibration values from today:

Date	ZeroPoint	Slope	pH4	pH7	pH10	Temp	Status	Notes
20250819	7.016	-56.26	170.1	-1.1	-171.5	19.1	OK	20250714_PP

CRM titration

Below are the resulted 2 CRM values from titrations.

Date	CRM value	Batch value	% off	Batch #	Status	Notes
20250819	2219.707	2224.47	-0.214112126	180	OK	CRM180_opened20250714_PP
20250819	2213.661	2224.47	-0.485922939	180	OK	CRM180_opened20250714_PP

Sample Titrations

-Below are the results from sample titrations that includes 5 samples.

Date	Sample ID	TA	dKH	Mass	Salinity	Status	Notes
20250819	BT 1	1688.344	4.7	60.047	35.14	OK
20250819	BT 2	1685.187	4.7	59.805	35.14	OK
20250819	BT 3	1686.201	4.2	59.957	35.13	OK
20250819	BT 4	1679.884	4.2	60.221	35.130	OK
20250819	DT 1	1740.094	4.0	60.13	35.63	OK

Overall observations: The titrations went all well however the expected TA values we got from the titrations is lowest since we have been dosing them with the Alkanlinity Reagent.

Other conclusions: Due to this, ZD and I checked if it is dosing right and recalibrated the Jebao and increased the volume of dosage for alkalinity from 15mls to 18mls with the previous four times a day interval. I would be following up with the checker regularly as well as with an upcoming titrations in next two weeks to see if it is going up. ZD thinks one of the reason this result is noticed is because of the corals using up the nutrients we are dosing and they are super excellent in health.