This protocol is based on the post originally made by Federica Scucchia’s notebook post.

Preparing Coral Homogenate for Physiological Analyses for the Bermuda ENCORE project

Protocols used

• Airbrushing protocol

I followed the above protocol to separate the tissue from the skeleton, which will then be used for surface area measurements using the wax-dipping method. The fragments belong to the species Madracis decactis (Mdec), Diploria labyrinthiformis (Dlab), Montastraea cavernosa (Mcav), and Porites astreoides (Past).

I divided the tissue homogenate into aliquots as follows (aliquots were then stored at -80°):

• Chlorophyll - 1.5 ml
• Symbiont Density - 1.5 ml
• Protein - 1.5 ml
• Lipids - 1.5 ml
• Carbohydrates - 1.5 ml
• Ashe-free dry weight - 5 ml
• Spare - 1 ml at least (if enough tissue)

Sample list

Here is the list of samples that were processed: